Riboflavin

Riboflavin

Synonyms Vitamin B2; Lactoflavin; Vitamin G; 7,8-Dimethyl-10- (D-ribo-2,3,4,5- tetrahydroxypentyl) isoalloxazine; 7,8-Dimethyl-10-ribitylisoalloxazine

CAS Number: 83-88-5 Molecular Formula: C17H2ON4O6 Molecular Weight: 376.369 g/mol

Beilstein Registry Number: 97825 EC Number: 201-507-1

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SKU 02102813-CF
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Description

Product Description

Riboflavin

Application Notes

Used as a photoinitiator for polymerization of polyacrylamide gels. It forms free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for electrophoresis. Riboflavin is commonly used in the stacking gel for non-denaturing polyacrylamide electrophoresis because native proteins can be senstive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerization until the gel is illuminated.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Specifications
SKU 02102813-CF
Application Notes Used as a photoinitiator for polymerization of polyacrylamide gels. It forms free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for electrophoresis. Riboflavin is commonly used in the stacking gel for non-denaturing polyacrylamide electrophoresis because native proteins can be senstive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerization until the gel is illuminated.
Beilstein Registry Number 97825
EC Number 201-507-1
Format powder
Grade USP Grade
Loss on Drying ≤1.5%
Melting Point 280 dec °C
Molecular Formula C17H2ON4O6
Molecular Weight 376.369 g/mol
Optical Rotation (c = 0.5, 0.05 M NaOH)
Pack Size No
Particle Size -115° to -135°
pH pH of saturated aqueous solution: about 6
Physical Appearance Orange Powder
Product Families Description Riboflavin
Product Overview Riboflavin
RTECS Number VJ1400000
Safety Symbol ≤0.3%
Solubility <h5 class="minor-section-title">Water Solubility</h5>84.7 mg/L (at 25 °C)
Source Soluble in 0.1 M Sodium Hydroxide (50 mg/ml - clear; yellowish-orange solution); only slightly soluble in water.
Storage and Handling Room Temperature
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.