Dino

Phytohemagglutin-Leukoagglutinin

Phytohemagglutin-Leukoagglutinin

Synonyms PHA-L; Phaseolus vulgaris agglutinin; PHA; PHA-P, Lectin from Phaseolus vulgaris

CAS Number: Not yet assigned

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SKU 02151886-CF
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Description

Product Description

Phytohemagglutin-Leukoagglutinin

Application Notes

Phytohemagglutin is very valuable in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Reconstitute with water to a final concentration of 25 mg/mL (0.2 mL water for the 5 mg size). This concentration of the lectin has been used for iontophoresis and neuron transport.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Mitogenic activity||Cell Biology||Cell Signaling and Neuroscience

Specifications
SKU 02151886-CF
Application Notes Phytohemagglutin is very valuable in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Reconstitute with water to a final concentration of 25 mg/mL (0.2 mL water for the 5 mg size). This concentration of the lectin has been used for iontophoresis and neuron transport.
Biochemical Physiological Actions PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Format lyophilized powder
Hazard Statements H317
Applications Mitogenic activity||Cell Biology||Cell Signaling and Neuroscience
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance White Lyophilized Powder
Product Families Description Phytohemagglutin-Leukoagglutinin
Product Overview Phytohemagglutin-Leukoagglutinin
Source Phaseolus vulgaris (Red Kidney Bean)
Specificity Carbohydrate Specificity: PHA-L recognizes a complex branched chain oligosaccharide structure.
Storage and Handling -20 °C
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.