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(TMB), (3,3',5,5' Tetramethylbenzidine)

(TMB), (3,3',5,5' Tetramethylbenzidine)

CAS Number: 54827-17-7 Molecular Weight: 240,35

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SKU 0898050-CF
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Description

Product Description

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Application Notes

TMB is a substrate for horseradish peroxidase. Develops a soluble blue reaction product that may be read at 370 or 655 nm. For endpoint assays the reaction may be stopped with acid, forming a yellow reaction product that may be read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Key Applications

ELISA

Specifications
SKU 0898050-CF
Application Notes TMB is a substrate for horseradish peroxidase. Develops a soluble blue reaction product that may be read at 370 or 655 nm. For endpoint assays the reaction may be stopped with acid, forming a yellow reaction product that may be read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Formulation Details Powder
Applications ELISA
Molecular Weight 240,35
Physical Appearance Cream powder
Product Families Description (TMB Powder)This product is excellent for ELISA procedures.C16H20N2 MW 240.3
Storage and Handling Store dry at room temperature. Protect from light.