Dino

Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine without Sodium Carbonate

Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine without Sodium Carbonate

Packaging size: 10 L

SKU 091003122
Price:
€25.80
In Stock
Request a quote
Description

Product Description

Basal Medium Eagle (BME) is one of the most commonly used of all cell culture media. This medium is the result of numerous studies from the late 1950's analyzing the nutrient requirements and other components essential for the growth of cells in culture. Traditionally, BME has been used in studies to measure the growth response of normal (WI-38) and transformed (HeLa) cells in monolayer culture. BME may be used for a broad variety of cell lines when properly supplemented.

Key Applications

Bacteria Cell Culture, Mammalian Cell Culture

Specifications
SKU 091003122
Application Notes
Formulation Details Powder | Polypeptone 10.0; Biotryptase 10.0; Heart Peptone 3.0; Corn Starch 1.0; Sodium Chloride 5.0; Agar 13.5; Final pH 7.3 +/- 0.2
Applications Bacteria Cell Culture, Mammalian Cell Culture
Pack Size 10 L
pH 6.5 ± 0.5 (w/o sodium bicarb.), 7.3 ± 0.5 (adjusted with sodium bicarb.)
Physical Appearance Orange Powder
Preparation Method Suspend 42.5 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Dispense and sterilize in an autoclave at 121 °C (15 lbs. psi) for 15 minutes. Cool to 45-50 °C and add 5-10% sterile defibrinated sheep or rabbit blood if desired.
Presentation Polypeptone 10.0; Biotryptase 10.0; Heart Peptone 3.0; Corn Starch 1.0; Sodium Chloride 5.0; Agar 13.5; Final pH 7.3 +/- 0.2 | Powder
Storage and Handling Store at +4 °C