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TRANSFORM & GROW BACTERIAL TRA

TRANSFORM & GROW BACTERIAL TRA

Synonyms Bacterial Transformation Kit

Packaging size: 50 Tests

SKU 112150200
Price:
€70.35
In Stock
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Description

Product Description

The Transform & Grow™ Bacterial Transformation Kit is for rapid and user-friendly preparation of transformation-competent bacterial cells.

Application Notes

In addition to the unique Transform-It™ Solution and MP Bio's SUPER-COMP Media, the Transform & Grow™ Bacterial Transformation Kit includes Roll & Grow™ Plating Beads to replace the traditional hockey stick and flame method for plating cells on solid growth media. The sterile Roll & Grow™ Plating Beads are added directly to the growth plate surface with the transformed bacterial cells, rolled back and forth to disperse the cells, and then discarded prior to incubation.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Transformation of bacterial cells

Specifications
SKU 112150200
Application Notes In addition to the unique Transform-It™ Solution and MP Bio's SUPER-COMP Media, the Transform & Grow™ Bacterial Transformation Kit includes Roll & Grow™ Plating Beads to replace the traditional hockey stick and flame method for plating cells on solid growth media. The sterile Roll & Grow™ Plating Beads are added directly to the growth plate surface with the transformed bacterial cells, rolled back and forth to disperse the cells, and then discarded prior to incubation.
Cells Tested E. coli
Components SUPER-COMP Media 120 ml |Transform-It™ Solution 6 ml |Roll & Grow™ Plating Beads 1 each
Media Type Competent Bacterial Media
Format Single Reagent Kit plus growth media and plating tools
Applications Transformation of bacterial cells
Kit Compatibility GENECLEAN® Kits |MERmaid™ Kits |FASTDNA™ Kits |FASTRNA™ Kits | RapidPURE™ Plasmid Mini Kits |PhoenIX™ Plasmid Kits |Other suppliers' kits
Kit or Assay Type Transformation Kits
Materials Required Not Supplied LB (Luria-Bertani) Broth or CIRCLEGROW® liquid culture media |5 ml sterile culture tubes for growth of the overnight bacterial culture |50 ml or larger sterile culture tubes or flasks for growth of diluted overnight culture |1.5 or 2.0 ml microcentrifuge tubes for the transformation reaction |15 ml sterile aerated polypropylene culture tubes for cell recovery |Agar plates for bacterial culture |37°C environmental shaking incubator |Pipettmen and tips |Ice |Spectrophotometer
Pack Size 50 Tests
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution |Encapsulated Media |Glass beads
Presentation Kit
Product Overview The Transform & Grow™ Bacterial Transformation Kit provides a simple and efficient method for the preparation, transformation and storage of competent E. coli cells. The transformation technique, based on observations by Chung et al., grows bacterial cells at 37°C, harvests them in mid-log growth and resuspends them in Transform-It™ Solution. This unique buffer both enables the cells to become immediately available for transformation and permits freezing the cells for future use without further preparation. The competent cells remain stable at -70°C to -80°C for at least 10 weeks. The Transform & Grow™ Bacterial Transformation Kit eliminates the requirement for heat shock and prepares competent cells within two and a half hours with only 30 minutes of hands-on manipulation. The kit contains the necessary reagents to prepare competent cells with a reliable and reproducible transformation efficiency of 2 - 8 x106 transformants per μg of supercoiled plasmid DNA.
Recommended Alternate Agrobacterium Transformation Kit
Recommended Replacement For 113301100
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Oncology
Sample Type Bacterial cells
Storage and Handling Store at Room Temperature (15-30 °C).
Unit Definition 2-8 x 106 transformants/µg of supercoiled plasmid DNA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.