FastGlycoProtein Isolation Kit

FastGlycoProtein Isolation Kit

Synonyms FastGlycoProtein™ Isolation Kit WGA Resin

Packaging size: 1 Kit

SKU 116550900
Price:
€333.40
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Specifications
SKU 116550900
FastPrep Instruments Models QuickPrep (24x2 mL); HiPrep (48x2 mL); CoolPrep (24x2 mL); QuickFlex (96x2 mL); ConeFlex Legacy (allows all FP24 adapters on FP96).
Application Notes Used to isolate Glycoprotein from complex protein mixtures including animal and plant tissue, cultured cells as well as microbe and insect, using the lectin Wheat Germ Agglutinin (WGA) immobilized on agarose.
Capacity Samples containing up to 1.5 mg protein
Components Lysing Matrix A 10 x2 mL Tubes, Garnet matrix and 1/4 inch ceramic spheres, WGA Lectin Resin 1.1 mL settled, as slurry, Binding/Wash Buffer. 5X solution 6.5 mL, Elution Solution 5 mL, Spin Filter with caps 10 each, Catch Tubes 20 each.
Format bead beating or chemical lysis
Hardness Zirconium sphere, Rockwell A 80-84, Vickers 1050; Garnet Flakes, Mohs 7.5-8
Applications Used to isolate Glycoprotein from complex protein mixtures including animal and plant tissue; cultured cells as well as microbe and insect; using the lectin concanavalin A (ConA) immobilized on agarose
Kit or Assay Type Affinity resin with Spin Columns
Material of Construction Matrix A, Garnet matrix and 6.35 mm ceramic spheres
Materials Required Not Supplied FastPrep® Instrument or alternative high speed disruptor; Microcentrifuge capable of 10,000 xg rcf that can freely spin 2.0 mL tubes; Microcentrifuge tubes (2.0 mL and 1.5 mL); Rotator or low-speed vortex; sterile water.
Molecule Enriched Glycoprotein
Pack Size 1 Kit
Product Overview An optimized Lysing Matrix A, coupled with any FastPrep® Instrument, quickly lyses most tissue samples in 40 seconds or less. Following lysis, samples are loaded to the WGA lectin resin SPIN filter tubes. The resin is washed and the Glycoproteins are eluted with the elution buffer. Eluted Glycoproteins are ready for 1-D gel electrophoresis and total protein (Bradford type) Assays. Other downstream applications may require sample processing to remove incompatible substances in the elution buffer.
Research Areas Immune regulation, Inflammation, Cell-to-cell adhesion and contact inhibition, Cell signaling, Protection against proteolytic degradation, and other biological processes
Sample Throughput 1-10 2mL Tubes
Sample Type animal and plant tissue; cultured cells as well as microbe and insect
Storage and Handling All FastGlycoProtein™ Isolation Kit; WGA Resin should be stored at 4°C immediately upon receipt. The Kit is shipped ambient. The kit reagents are guaranteed for up to one year from the date of purchase of the kit.
Tube Compatibility 2 ml
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.