FastDNA™ Spin Kit for Soil

FastDNA™ Spin Kit for Soil

Synonyms FastDNA Soil Kit

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SKU 116560200-CF
Price:
€312.25
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Description

Product Description

The FastDNA Spin Kit for Soil is designed to isolate bacterial, fungal, plant, and animal genomic DNA from soil and other environmental samples. Soil is processed by a FastPrep® instrument and Lysing Matrix E tubes designed to efficiently lyse all microorganisms. The released DNA is purified by a silica-based spin filter method and is suitable for PCR analysis and other downstream applications. Complete lysis of all organisms, including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi is achieved within seconds using a FastPrep instrument and Lysing Matrix E tubes.

Application Notes

Cited in over 6000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.

Specifications
SKU 116560200-CF
FastPrep Instruments Models QuickPrep (24x2 mL); HiPrep (48x2 mL); CoolPrep (24x2 mL); QuickFlex (96x2 mL); ConeFlex Legacy (allows all FP24 adapters on FP96)
Alternate Names FastDNA Soil Kit
Application Notes Cited in over 6000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors
Brand FastDNA™
Certifications DNase and RNase free Lysing Matrix tubes
Components 50 x 2.0 mL tubes of Lysing Matrix E, 60 mL Sodium Phosphate Buffer, 8 mL MT Buffer, 25 mL of PPS Solution, 66 mL Binding Matrix, 50 Spin Modules, 50 Catch Tubes, 12 mL Concentrated SEWS-M, 20 mL DES, 200 µl BBS Gel Loading Dye
Hardness Ceramic Spheres, Vickers 800; Silica Spheres, Knoop 515; Glass Bead, Knoop 515
Applications Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
Material of Construction Matrix E, 1.4 mm ceramic spheres, 0.1 mm silica spheres, and one 4 mm glass bead
Materials Required Not Supplied FastPrep instrument to lyse samples, Microcentrifuge to freely spin 2.0 mL tubes, Microcentrifuge tubes (2.0 mL and 1.5 mL), Clean 15 mL tubes for DNA binding; Rotator or low-speed vortex
Matrix Type E
Molecule Enriched DNA
Pack Size No
Personal Protective Equipment If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (Labcoat and long-sleeves), and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices.
pH Reagent: SEWS-M pH Range: 6.4-6.8
Research Areas Soil & Environmental Science
Sample Type Soil, Plants, Animal Tissues, Feces, Yeast, Bacteria (gram+/-), Fungi, Spores, Wastewater, Food, Seeds, Roots
Sample Volume up to 500 mg per prep
Specific Gravity Ceramic Spheres, 4; Silica Spheres, 2.5; Glass Bead, 2.5
Storage and Handling All FastDNA Spin Kit for Soil reagents are shipped ambiently. FastDNA Spin Kit for Soil reagents are stable at room temperature. (15-30 ° C).
Tube Compatibility 2 mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.