Dino

Buffers

Buffers

Buffers maintain their pH when small amounts of strong acids or bases are added. In biology, buffer solutions are used to provide a constant pH environment for biochemical processes. The pH often changes the environments of tissues and cells and can alter the activity of biomolecules. Buffers maintain the correct pH for proteins and oligonucleotides, and are especially important for techniques like electrophoresis.

Commonly used buffers in life science research share some of these characteristics:

  • A pKa value between 6.0 and 8.0
  • High water solubility
  • Minimal changes due to temperature and concentration
  • Limited effects due to ionic or salt composition of the solution

Browse our comprehensive selection of buffers including common buffers tris(hydroxymethyl)aminomethane (tris) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES).

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Tris-Glycine Buffer

Tris-Glycine Buffer

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Magnesium Chloride

Magnesium Chloride

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Phenol, Saturated Solution, Ph 4.5

Phenol, Saturated Solution, Ph 4.5

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Phenol : Chloroform Saturated Solution, Ph 5.2

Phenol : Chloroform Saturated Solution, Ph 5.2

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Phenol:Chloroform Saturated Solution, Ph 6.7

Phenol:Chloroform Saturated Solution, Ph 6.7

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TAE 50X BUFFER--N/H

Buffer for Electrophoresis.

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Urea, Ultra-Pure (1lb Size)

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

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Formamide Deionized

Deionized Formamide is used in molecular biology and a denaturing agent for nucleic acids in gel electrophoresis orr hybridization experiments. In the latter case the role of formamide is to decrease the temperature necessary for the reassociation of complementary nucleic acids.

GLYCOGEN

Glycogen is an inert material and does not interfere with molecular biology experiments. It can replace tRNAs or sonicated DNAs as an additive for the precipitation of nucleic acids.