Culture Media, Liquid
Dulbecco's Modification Of Eagle's Medium With L-Glutamine and 4.5 g/L Glucose
Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP. S
Earle's Balanced Salts (10X Solution) Without Sodium Bicarbonate
Earle's Balanced Salts (10X Solution) without sodium bicarbonate
Minimum Essential Medium Eagle With Earle's Salts and 2.0 g/L Sodium Bicarbonate Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine
MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.
Ham's F-10 (10X Solution) Without L-Glutamine, Without Sodium Bicarbonate, With 12.4 mg/L Phenol Red
Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.
Ham's F12 (1X Solution) With L-Glutamine
Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konig
1X Hanks' Balanced Salt Solution (Modified) Without Phenol Red
Inorganic Salts: Calcium Chloride [CaCl2] 140.0000 mg/L, Magnesium Sulfate [MgSO4] 97.7000 mg/L, Potassium Chloride [KCl] 400.0000 mg/L, Potassium Phosphate Monobasic [KH2PO4] 60.0000 mg/L, Sodium Bicarbonate [NaHCO3] 350.0000 mg/L, Sodium Chloride [NaCl] 8000.0000 mg/L, Sodium Phosphate Dibasic [Na2HPO4] 47.7000 mg/L, Dextrose 1000.0000 mg/L
1XHanks' Balanced Salts (Modified), Without Magnesium and Calcium
1× Hanks' Balanced Salts (Modified), without Magnesium and Calcium
Hank's Balanced Salts (Hbs) (Modified) (1X Solution) Without Calcium, Magnesium, Phenol Red
Hank's Balanced Salts (HBS) (Modified) (1X Solution) without calcium, magnesium, phenol red
High Gem (Growth Enhancement Medium) (1X Solution) Without L-Glutamine
High Growth Enhancement Medium, without L-Glutamine is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.
Human Type Ab Serum (HCV, HIV, HbsAG Free), Heat Inactivated
This serum is collected from Type AB human blood.
Leibovitz L-15 Medium (Modified) (1X Solution) Without L-Glutamine
In this medium dextrose is replaced by galactose. In addition, buffering is provided by the free bases of the amino acids in place of sodium bicarbonate. As a result this medium has been used for growth of tissues in free gaseous exchange with the atmosphere.
1X Mccoy 5A Medium, Iwakata & Grace Modification With L-Glutamine
McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki. The Park and Terasaki Modification (PT) differs from the Iwakata and Grace Modication (IG) in that PT contains slightly modified levels of L-glutamic acid, glycine, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine. The PT modification also contains the additions of penicillin, dihydrostreptomycin sulfate, gentamycin, fetal bovine serum and HEPES.
MEM Amino Acids (50X Solution) Without L-Glutamine
In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc. As a result, the availability of a wide variety of media supplements is desirable when designing cell culture experiments.
Minimal Essential Medium Non-Essential Amino Acids (100X Solution)
100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.