Culture Media, Powder, Bacterial Media
Terrific Broth, Modified
Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.
Tryptic Soy Agar
Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.
Tryptic Soy Broth
Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.
Tryptone is a pancreatic digest of casein. Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the production of indol. This preparation supplements media for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as E. coli and Pseudomonas species. It is used to supplement media in the cultivation of anaerobes (Clostridia and other fermenting organisms, e.g., Lactobacilli).
Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine without Sodium Carbonate
One of the most commonly used of all cell culture media for a variety of cell lines.
Malt Extract Agar (MEA)
Malt Extract Agar has been used for years to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and other drinks. It is also recommended in conjunction with other specific media which are included in this manual. A medium for the isolation and enumeration of yeasts and molds. It can be used in cultivation of the strains for microbiological vitamin assays.
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.
Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.