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Culture Media, Single Reagent Kit plus DNA

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Casein Hydrolysate Acid Solution

Casein Hydrolysate Acid Solution

Follicle Stimulating Hormone

Follicle Stimulating Hormone

Alsever's Solution

Alsever’s solution is an isotonic, balanced salt solution.

Basal Medium Eagle (BME) Vitamin Concentrate (100X)

100X BME, Modified
Vitamins
Without L-glutamine
Shipped frozen with dry ice.

Brain Heart Infusion Broth

Brain Heart Infusion Broth is a modification of the media by Rosenow and Hayden. The infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer.

10X Trypsin HBSS Without Calcium and Magnesium

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Basal Medium Eagle (modified) with Hank's Salts (HBME) with L-glutamine without Sodium Carbonate

One of the most commonly used of all cell culture media for a variety of cell lines.

Dulbecco's Modification Of Eagle's Medium (1X Solution) With 4.5 g/L Dextrose, Without L-Glutamine and Inositol

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP

Dulbecco's Modification Of Eagle's Medium (1X Solution) Without L-Glutamine, Leucine, Sodium Pyruvate

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

Dulbecco's Modification Of Eagle's Medium (DMEM) (1X Solution) Without L-Glutamine, Phenol Red

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate

Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate

Dulbecco's Modification Of Eagle's Medium With L-Glutamine and 4.5 g/L Glucose

Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available from MP. S

Earle's Balanced Salts (10X Solution) Without Sodium Bicarbonate

Earle's Balanced Salts (10X Solution) without sodium bicarbonate

Earle's Balanced Salts (Modified) (EBS) (1X Solution) Without Phenol Red

Earle's Balanced Salts (Modified) (EBS) (1X Solution) without phenol red

Earle's Balanced Salts Without Sodium Bicarbonate

Earle's Balanced Salts without Sodium Bicarbonate. Particle Size: >90% thru 100 mesh pH (with 2200 mg/L NaHCO3): 7.57 Osmolality (with 2200 mg/L NaHCO3): 289 mM/kg Endotoxin: <0.10 EU/ml Mycoplasma: Negative

EY Tellurite Enrichment

Egg Yolk Tellurite Enrichment is a tellurite egg yolk emulsion. To be used with Baird-Parker Agar Base for isolating S. aureus.

Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 0.35 g/L Sodium Bicarbonate Without L-Glutamine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Minimum Essential Medium Eagle (Modified) (1X Solution) With Hank's Salts, 20 mM HEPES, Without L-Glutamine, Sodium Bicarbonate

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Minimum Essential Medium Eagle With Earle's Salts and 2.0 g/L Sodium Bicarbonate Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Fetal Bovine Serum (FBS), South American Origin

Due to its high level of nutrients, this FBS is the growth supplement of choice for cell culture.

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Glycerol, Microbiological Grade

Microbiological Grade
Highly purified for use as a fixative, a component in bacterial preservation media and for the isolation and maintenance of a variety of microorganisms.

High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate

High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Ham's F-10 (10X Solution) Without L-Glutamine, Without Sodium Bicarbonate, With 12.4 mg/L Phenol Red

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

Ham's F12 (1X Solution) With L-Glutamine

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konig

1X Hanks' Balanced Salt Solution (Modified) Without Phenol Red

Inorganic Salts: Calcium Chloride [CaCl2] 140.0000 mg/L, Magnesium Sulfate [MgSO4] 97.7000 mg/L, Potassium Chloride [KCl] 400.0000 mg/L, Potassium Phosphate Monobasic [KH2PO4] 60.0000 mg/L, Sodium Bicarbonate [NaHCO3] 350.0000 mg/L, Sodium Chloride [NaCl] 8000.0000 mg/L, Sodium Phosphate Dibasic [Na2HPO4] 47.7000 mg/L, Dextrose 1000.0000 mg/L

1X Hanks' Balanced Salt Solution, Modified

1× Hanks' Balanced Salt Solution, Modified

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1XHanks' Balanced Salts (Modified), Without Magnesium and Calcium

1× Hanks' Balanced Salts (Modified), without Magnesium and Calcium

Hank's Balanced Salts (Hbs) (Modified) (1X Solution) Without Calcium, Magnesium, Phenol Red

Hank's Balanced Salts (HBS) (Modified) (1X Solution) without calcium, magnesium, phenol red

High Gem (Growth Enhancement Medium) (1X Solution) Without L-Glutamine

High Growth Enhancement Medium, without L-Glutamine is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Human Type AB Serum (HCV, HIV, HbsAG Free), Heat Inactivated

This serum is collected from Type AB human blood.

Human Type AB Serum From Male Donors

This serum is collected from Type AB human blood.

Human Type AB Serum (HCV, HIV, HbsAG Free)

This serum is collected from Type AB human blood.

Human Serum, Pooled (HCV, HIV, HBsAG Free)

This is pooled human serum obtained from normal, clotted blood.

Lamb Serum

This serum is derived from whole blood of healthy young lambs.

Leibovitz L-15 Medium (Modified) (1X Solution) Without L-Glutamine

In this medium dextrose is replaced by galactose. In addition, buffering is provided by the free bases of the amino acids in place of sodium bicarbonate. As a result this medium has been used for growth of tissues in free gaseous exchange with the atmosphere.

Malt Extract Agar (MEA)

Malt Extract Agar has been used for years to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and other drinks. It is also recommended in conjunction with other specific media which are included in this manual. A medium for the isolation and enumeration of yeasts and molds. It can be used in cultivation of the strains for microbiological vitamin assays.

1X Mccoy 5A Medium, Iwakata & Grace Modification With L-Glutamine

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki. The Park and Terasaki Modification (PT) differs from the Iwakata and Grace Modication (IG) in that PT contains slightly modified levels of L-glutamic acid, glycine, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine. The PT modification also contains the additions of penicillin, dihydrostreptomycin sulfate, gentamycin, fetal bovine serum and HEPES.

Mccoy's 5A Medium (Park & Terasaki Modification) (1X Solution) Without L-Glutamine, Sodium Bicarbonate

McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki.

MEM Amino Acids (50X Solution) Without L-Glutamine

In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc. As a result, the availability of a wide variety of media supplements is desirable when designing cell culture experiments.

Minimal Essential Medium Non-Essential Amino Acids (100X Solution)

100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.

Minimum Essential Medium Eagle (Modified) With Earle's Salts, L-Glutamine, Non-Essential Amino Acids, Without Sodium Bicarbonate

(MEM Powder, Modified)
With Earle's salts, non-essential amino acids, and L-glutamine
Without sodium bicarbonate

Murashige and Skoog Basal Salt Medium With Gamborg's Vitamins

Includes the macro- and mironutrients as described by Murashige and Skoog, 1962, and vitamins as described by Gamborg, et al., 1966.

Murashige and Skoog's Modified Vitamin Solution (1000X)

Includes the vitamins as described by Murashige and Skoog, 1962. Use at a concentration of 1 ml/L medium.

Nutrient Broth

Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.

10X Dulbecco's Phosphate Buffered Saline, Without Calcium and Magnesium

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

Pluronic® F-68 Polyol

Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. It is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood.

Pluronic® F-68 Polyol, 10% Solution, (100×)

Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. It is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood.

Potato Dextrose Agar

Suited for the cultivation of yeasts and molds from dairy products and other foods. It may also be used to induce sporulation in many fungi.

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Potato Dextrose Broth

Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and molds. It can be used in the analysis of dairy products, bottled drinks, frozen food and other types of food. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of mirocultivation in slides. When the medium is to be used for enumeration of molds and yeasts, add to the medium, sterilized and cooled to 45-50 °C, approximately 14 mL of a sterilized 10% solution of tartaric acid to obtain a pH of 3.5.

Pseudomonas Agar F

Used with glycerol (cat. no. 1004417) for the isolation, cultivation and differentiation of Pseudomonas aeruginosa on the basis of phycocyanin (pigment) production.

1X RPMI Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine and Phosphate, With 0.85 g/L Sodium Bicarbonate

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

1X RPMI 1640 Without L-Glutamine, Phenol Red

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. Without L-glutamine, phenol red; Endotoxin Tested; Sterile Filtered

RPMI 1640 (1X Solution) Without L-Glutamine and L-Leucine

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

RPMI 1640 Without L-Glutamine

RPMI 1640 (1X Solution) without L-glutamine, pH 6.9 to 7.2. This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

Sabouraud Dextrose Agar Without Chloramphenicol

Sabouraud Dextrose Agar can be used for the isolation, identification and maintainance of pathogenic and saprophytic fungi. It is suited for the cultivation of yeasts, molds and aciduric microorganisms.

Mp-Mrc-5 Serum-Free Cell Culture Medium

MP-MRC-5® Medium, Serum Free, very low protein (90 ng/mL) medium developed specifically for the culture of MRC-5 cells. MP-MRC-5™ medium was developed specifically for microcarrier culture of MRC-5 cells in production systems. It is completely animal component-free and contains very little protein. MP-MRC-5™ achieves growth rates superior to standard serum-supplemented media. Adaptation from serum-supplemented media is not necessary.

TCH® Defined Serum Replacement, 50× Concentrate

TCH is a fortified, multipurpose serum replacement for long-term culturing of primarily human anchorage-dependent and suspension cells. TCH® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.

TCM® Defined Serum Replacement

TCM™ is a fortified, multipurpose serum replacement for long-term culturing of many types of anchorage dependent and suspension cultures. TCM® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.

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100 mM Sodium Pyruvate (11.00 mg/mL)

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.

Chicken Embryo Extract 20 mL

Chicken embryo extract is prepared from 9-12 day old eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.

Hat Supplement (50X Solution)

(50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

HT (50X Solution)

(50X Concentrate) Hypoxanthine 13.61 mg/L Thymidine 3.876 mg/L This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

ITS Premix Solution

ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle's balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.

Supplement VX

Supplement VX is recommended as an enrichment for the cultivation of Neisseria gonorrhoeae, Haemophilus influenzae and other fastidious organisms. Supplement VX is a sterile lyophilized concentrate of essential growth factors for supplementing media for culturing microorganisms with exacting growth requirements. It is used for the preparation of chocolate agar from GC Agar Base and hemoglobin for isolation and cultivation of N. gonorrhoeae. It is also recommended for enriching Brain Heart Infustion Agar and Brain Heart Infusion Broth

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Terrific Broth

Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.

Terrific Broth, Modified

Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.

Trace Element Solution 1 (1000×)

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

Trace Element Solution 2 (1000×)

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I.

Tryptic Soy Agar

Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.

Tryptic Soy Broth

Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.

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Tryptone

Tryptone is a pancreatic digest of casein. Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the production of indol. This preparation supplements media for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as E. coli and Pseudomonas species. It is used to supplement media in the cultivation of anaerobes (Clostridia and other fermenting organisms, e.g., Lactobacilli).

Tryptose Phosphate Broth

Tryptose Phosphate Broth is a buffered medium used for cultivating fastidious microorganisms. Tryptose phosphate broth has been used for testing the sensitivity of microorganims to antibiotics by the tube dilution method. Waisbren, Carr and Dunnett demonstrated that a soy bean peptone medium inhibited the action of neomycin, Aureomycin®, terramycin and polymyxin against the test organism while tryptose phosphate broth, dextrose broth and nutrient broth were suitable for the test.

Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate

Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.

10 X RB CA TXN SALTS

Ready-to-Use solution, 10x concentrate

Agar-B, 454 g

Bulk powder, easily weighed to make custom batch sizes.

ALKALI-CATION YEAST TRANSFORMA

The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.

BSM-HIS-LEU-TRP-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CASEIN PEPTONE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CIRCLEGROW (STERILE SOLUTION)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

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CSM-ADE-HIS-LEU-LYS-TRP-URA (Powder)

Bulk powder, easily weighed to make custom batch sizes.

CSM-ADE-HIS-LEU-MET-TRP-URA (P

Bulk powder, easily weighed to make custom batch sizes.

CSM-ADE-HIS-LEU-TRP-URA (POWDE

Bulk powder, easily weighed to make custom batch sizes.

CSM-ADE-LEU (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-ADE-TRP-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-HIS-LEU-LYS-TRP-URA (POWDE

Bulk powder, easily weighed to make custom batch sizes.

CSM-HIS-LEU-TRP W/40 MG/L ADE

Bulk powder, easily weighed to make custom batch sizes.

CSM-LEU W/40 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-LEU-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-TRP W/20 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-URA W/20 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

CSM-URA W/40 MG/L ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

DOB 2% GALACTOSE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

DOB 2% GALACTOSE, 1% RAFFINOSE

Bulk powder, easily weighed to make custom batch sizes.

DOB 2% RAFFINOSE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

DOB 2% RAFFINOSE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

DOBA W/ 2% GALACTOSE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

EMM (PACK)(POUCHES)

Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.

EMM NO NITROGEN (NO NH[4]CL, G

Bulk powder, easily weighed to make custom batch sizes.

EZ-YEAST TRANSFORMATION KIT

- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA

HL5 MEDIUM (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

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LB-AGAR, LENNOX (LARGE CAPSULE

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

LB-AGAR, LENNOX (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

M9 MINIMAL MEDIUM SALTS (PART

Bulk powder, easily weighed to make custom batch sizes.

NZY-HARVARD BROTH (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

PEPTONE-Y (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC AMINO ACID MIXTURE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-ADE (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-ADE-HIS-LEU-LYS-TRP-URA (PO

Bulk powder, easily weighed to make custom batch sizes.

SC-ADE-HIS-LEU-TRP (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-CYS-MET (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-HIS (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-HIS-LEU-TRP (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-HIS-LEU-TRP-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-LEU (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-LEU-TRP (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-LEU-TRP-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-LEU-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-LYS (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-TRP (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SC-TRP-URA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SD MEDIUM-TRP (PACK)(POUCHES)

Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.

SD MEDIUM-TRP (PACK)(POUCHES)

Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.

SDA MEDIUM-ADE-HIS-LEU-TRP (PA

Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.

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SOB (CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

SOB (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

SOC (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

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SUPERBROTH (CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

TERRIFIC BROTH PART 1 (CAPSULE

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

TERRIFIC BROTH PART 1 (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

TRANSFORM & GROW BACTERIAL TRA

- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time

YEP BROTH (LARGE CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

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YEP BROTH (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

YES AGAR (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

YNB W/AMMONIUM SULFATE, W/O PH

Bulk powder, easily weighed to make custom batch sizes.

YNB W/O AMMONIUM SULFATE, W/O

Bulk powder, easily weighed to make custom batch sizes.

YNB W/O AMMONIUM SULFATE, W/O

Bulk powder, easily weighed to make custom batch sizes.

YNB W/ AMMONIUM SULFATE, W/O I

Bulk powder, easily weighed to make custom batch sizes.

YNB W/O AMMONIUM SULFATE, W/O

Bulk powder, easily weighed to make custom batch sizes.

YNB W/O AMMONIUM SULFATE, W/O

Bulk powder, easily weighed to make custom batch sizes.

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YPD (YEPD) Broth (Large Capsules)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

YPD (Sterile Solution)

Ready-to-Use formulation

YT-BROTH AGAR (CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

YT-BROTH AGAR (CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

Casein hydrolysate enzymatic

Casein hydrolysate enzymatic is a pancreatic hydrolysate of casein. This hydrolysate contains, in the form of mixed amino acids and peptides, all amino acids originally present in casein. It is a refined product providing high quality soluble source of amino acids,polypeptides and peptides.

Casein Hydrolysate Acid

Casein Hydrolysate Acid

Yeast, Bakers Dried Active

Yeast, Bakers Dried Active

Soy Hydrolysate Enzymatic

Soy Hydrolysate Enzymatic

Yeast Extract Powder

Yeast Extract Powder

Yeast Hydrolysate Enzymatic

Yeast Hydrolysate Enzymatic

Alpha Modification Of Eagle's Medium (AMEM), Powder, With L-Glutamine, Without Deoxyribosides, Ribosides, Sodium Bicarbonate

Alpha Modification of Eagle's Medium (AMEM), powder, with L-glutamine, without deoxyribosides, ribosides, sodium bicarbonate. Originally used for growth of hamster kidney cells.

Ham's F-12 (Modified) With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells. MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.

Hanks' Balanced Salts, Powder, Without Sodium Bicarbonate

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Phosphate Buffered Saline (PBS), Dulbecco's Formula, Powder, Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Hoagland's Modified Basal Salt Mixture

Hoagland's Modified Basal Salt Mixture includes the macro- and micronutrients as described by Hoagland and Arnon, 1950.

Newborn Bovine Serum (NBS) 

This serum is produced from blood collected from newly born calves between 1-14 days of age.

Rabbit Serum

This serum is derived from the blood of healthy adult animals. Rabbit serum is used to support cultures of Borrelia burgdorferi, the causative agent of Lyme disease.

circlegrow-small-capsules-113000121

Circlegrow (capsules)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

CIRCLEGROW (POWDER)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

CIRCLEGROW (LARGE CAPSULES)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

CIRCLEGROW (PACK)(POUCHES)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

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L-BROTH (CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

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L-BROTH AGAR (CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

L-BROTH AGAR (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

LB-MEDIUM (LARGE CAPSULES)

Encapsulated Media: the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water! |- No weighing. No dust. No cleanup. No smell. - Simply drop capsules in water and autoclave - Unbeatable selection of formulations - Also available in other pre-measured formats

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LB Medium

No weighing. No dust. No cleanup. No smell. Simply drop capsules in water and autoclave.

LB MEDIUM (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

LB-MEDIUM (PACK)(POUCHES)

Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water.