Culture Media, Culture Media, Yeast Media
EZ-YEAST TRANSFORMATION KIT
- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA
ALKALI-CATION YEAST TRANSFORMA
The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.
Supplement VX is recommended as an enrichment for the cultivation of Neisseria gonorrhoeae, Haemophilus influenzae and other fastidious organisms. Supplement VX is a sterile lyophilized concentrate of essential growth factors for supplementing media for culturing microorganisms with exacting growth requirements. It is used for the preparation of chocolate agar from GC Agar Base and hemoglobin for isolation and cultivation of N. gonorrhoeae. It is also recommended for enriching Brain Heart Infustion Agar and Brain Heart Infusion Broth