Culture Media, Yeast Media
EZ-YEAST TRANSFORMATION KIT
- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA
ALKALI-CATION YEAST TRANSFORMA
The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.
Potato Dextrose Broth
Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and molds. It can be used in the analysis of dairy products, bottled drinks, frozen food and other types of food. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of mirocultivation in slides. When the medium is to be used for enumeration of molds and yeasts, add to the medium, sterilized and cooled to 45-50 °C, approximately 14 mL of a sterilized 10% solution of tartaric acid to obtain a pH of 3.5.