Culture Media

280 Results Found

RPMI 1640 Without L-Glutamine

RPMI 1640 (1X Solution) without L-glutamine, pH 6.9 to 7.2. This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

Sabouraud Dextrose Agar Without Chloramphenicol

Sabouraud Dextrose Agar can be used for the isolation, identification and maintainance of pathogenic and saprophytic fungi. It is suited for the cultivation of yeasts, molds and aciduric microorganisms.

Mp-Mrc-5 Serum-Free Cell Culture Medium

MP-MRC-5® Medium, Serum Free, very low protein (90 ng/mL) medium developed specifically for the culture of MRC-5 cells. MP-MRC-5™ medium was developed specifically for microcarrier culture of MRC-5 cells in production systems. It is completely animal component-free and contains very little protein. MP-MRC-5™ achieves growth rates superior to standard serum-supplemented media. Adaptation from serum-supplemented media is not necessary.

TCH® Defined Serum Replacement, 50× Concentrate

TCH is a fortified, multipurpose serum replacement for long-term culturing of primarily human anchorage-dependent and suspension cells. TCH® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.

TCM® Defined Serum Replacement

TCM™ is a fortified, multipurpose serum replacement for long-term culturing of many types of anchorage dependent and suspension cultures. TCM® Defined Serum Replacement shows following features: A universal serum replacement that supports human and mammalian cells; completely free of virus, mycoplasma and other infectious agents; contains no growth factors, steroid hormones, extraneous proteins or phenol red; protein origin - human; Drug Master on file with the FDA.

0916820.png

100 mM Sodium Pyruvate (11.00 mg/mL)

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.

Chicken Embryo Extract 20 mL

Chicken embryo extract is prepared from 9-12 day old eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.

Hat Supplement (50X Solution)

(50X Concentrate)Hypoxanthine 13.61 mg/LAminopterin 2H2O 0.1906 mg/LThymidine 3.876 mg/LSelective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

HT (50X Solution)

(50X Concentrate) Hypoxanthine 13.61 mg/L Thymidine 3.876 mg/L This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production.

ITS Premix Solution

ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle's balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.

Supplement VX

Supplement VX is recommended as an enrichment for the cultivation of Neisseria gonorrhoeae, Haemophilus influenzae and other fastidious organisms. Supplement VX is a sterile lyophilized concentrate of essential growth factors for supplementing media for culturing microorganisms with exacting growth requirements. It is used for the preparation of chocolate agar from GC Agar Base and hemoglobin for isolation and cultivation of N. gonorrhoeae. It is also recommended for enriching Brain Heart Infustion Agar and Brain Heart Infusion Broth

Terrific Broth

Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.

Terrific Broth, Modified

Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.

Trace Element Solution 1 (1000×)

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

Trace Element Solution 2 (1000×)

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I.

Tryptic Soy Agar

Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.

Tryptic Soy Broth

Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.

Tryptone

Tryptone is a pancreatic digest of casein. Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the production of indol. This preparation supplements media for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as E. coli and Pseudomonas species. It is used to supplement media in the cultivation of anaerobes (Clostridia and other fermenting organisms, e.g., Lactobacilli).

Tryptose Phosphate Broth

Tryptose Phosphate Broth is a buffered medium used for cultivating fastidious microorganisms. Tryptose phosphate broth has been used for testing the sensitivity of microorganims to antibiotics by the tube dilution method. Waisbren, Carr and Dunnett demonstrated that a soy bean peptone medium inhibited the action of neomycin, Aureomycin®, terramycin and polymyxin against the test organism while tryptose phosphate broth, dextrose broth and nutrient broth were suitable for the test.

Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate

Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.