Silver Enhancing Kit (For Blotting)
Silver Enhancing Kit is an easy to use and sensitive method for enhancement of immunogold labelled antigens on a solid support. It depends on the reduction of silver from solution in the presence of a developer by the gold label attached to proteins/nucleic acids mounted on a solid phase for direct viewing by eyes. The components of the solutions have been selected to allow good control over time of development with minimal background. Because of its relative insensitivity to light, the enhancement may be monitored in normal room lighting during development. The specimens need no fixing after development but may be thoroughly washed in tap water. The development operates at pH 7.4 to minimize the difficulties experienced with other enhancing methods where very low pH sometimes causes the extraction of labelled proteins from the specimen.
Immunodiffusion Plates, Uncalibrated, Unfilled
Immunodiffusion Plates are polystyrene with close-fitting covers to minimize gel dehydration. Plate bottoms are recessed 7 mm to prevent scratching on the bench surfaces. The plate is hydrophilic for easy gel pouring and better adherence to the plate.
Mouse IgM Rid Kit
Mouse IgM RID kit is used for quantitative determination of mouse IgM. Radial Immunodiffusion (RID) is a quantitative immunodiffusion technique used to determine the concentration of an antigen in a biological fluid. This method is based on the diffusion of an antigen in a radial pattern from a cylindrical well through an agarose gel containing an appropriate mono-specific antibody. As the antigen diffuses radially in all directions into the agarose, its concentration continuously decreases until the equivalence point is reached. At this point an immune precipitation occurs as the antigen and antibody reach equilibrium; the result is the formation of a precipitin ring. Since the diameter of the ring is directly proportional to the antigen concentration, a calibration curve of diameter versus antigen concentration can be constructed using a series of reference standards of known antigen concentration. The concentration of the antigen in an unknown sample is then determined by the measurement of the ring diameter produced by that unknown sample and reading the corresponding concentration values from the calibration curve.
Micro - Ouchterlony Plates
Each MP immunodiffusion plate contains 8 ouchterlony patterns consisting of a center well and 6 outer wells. The MP ouchterlony plate is designed for specific but low affinity antisera. Such antisera require properly spaced wells and polyethylene glycol in the agarose for optimal immuno-precipitation. The plates are prepared especially for the MP polyclonal antisera to human IgG subclasses. When using these antisera for typing immunoglobulin subclasses in patients with myeloma, the sera must be diluted so that the background IgG does not produce visible precipitation.
Each package contains 5 plates for use in typing procedures. The MP Ouchterlony Plates have eight (8) antigen wells (8mm diameter), each surrounded by six (6) antisera wells (3mm diameter). These plates have been especially designed for work involving antigens present in very low concentrations. The large antigen well allows 75 microliters of sample to be transferred at one time. The smaller antisera wells (3mm diameter) will hold a maximum of 12 microliters. The format is particularly useful for isotyping of tissue culture supernatants of monoclonal antibodies.